User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/10/01: Difference between revisions
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==, 2014== | ==October 1, 2014== | ||
===Procedures=== | ===Procedures=== | ||
====R6G Fluorescence: Calibration and Measurement==== | |||
#Make stock concentrations (both groups can use the same solutions) | |||
#*0.10uM | |||
#*0.50uM | |||
#*1.0uM | |||
#*1.5uM | |||
#*2.0uM | |||
#Take UV-Vis and Fluorescence spectra of these samples. | |||
#Make a calibration curve based on UV-Vis. | |||
#*Compare your data to some published values | |||
#Make a calibration curve based on the fluorescence. | |||
#*In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height. | |||
===Data=== | ===Data=== | ||
Revision as of 21:03, 30 September 2014
 CHEM 581: Experimental Chemistry
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October 1, 2014ProceduresR6G Fluorescence: Calibration and Measurement
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