User:Madeleine Y. Bee/Notebook/CHEM-581 Experimental Chemistry/2014/10/01

From OpenWetWare
Jump to navigationJump to search
CHEM 581: Experimental Chemistry <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

October 1, 2014

Procedures

R6G Fluorescence: Calibration and Measurement

  1. Make stock concentrations (both groups can use the same solutions)
    • Initial stock: 2500uM, secondary stock: 500uM
    • 0.10uM
      • Made with 500uM solution
    • 0.50uM
      • Made with 500uM solution
    • 1.0uM
      • Made with 500uM solution
    • 1.5uM
      • Made with 500uM solution
    • 2.0uM
      • Made with 2500uM solution
  2. Take UV-Vis and Fluorescence spectra of these samples
    • Settings:
      • 500nm excitation
      • 515-700nm scan range
      • 10.0nm slit width
      • 200nm/min scan speed
  3. Make a calibration curve based on UV-Vis.
    • Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    • In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.

Data


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/CHEM-581_Experimental_Chemistry/2014/10/01&oldid=825809"