User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/01/28: Difference between revisions
From OpenWetWare
(Autocreate 2015/01/28 Entry for User:Madeleine_Y._Bee/Notebook/CHEM-582_Experimental_Chemistry_II) |
No edit summary |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==January, 27, 2015== | ||
* | *First trial of synthesizing PVA-Alginate beads with and without NaMT | ||
==Protocol== | |||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | |||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C | |||
## 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) | |||
## ~0.1 g of nanocomposites should be added to this mixture where applicable | |||
# Cool dissolved and mixed solution to 35°C | |||
# Prepare 210 mL of saturated citric acid with 2% calcium chloride | |||
## Add 4.2 grams of calcium chloride to 210 mL of water | |||
## Add citric acid to saturation | |||
# Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle | |||
# Stir for 24hrs to allow solidification | |||
# Rinse with water to remove excess reactants | |||
Revision as of 16:20, 27 January 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
January, 27, 2015
ProtocolProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.>
|