User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/02/27: Difference between revisions
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===Procedure=== | ===Procedure=== | ||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)]<br.> | Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.> | ||
<br.> | <br.> | ||
PVA-Alg-NaMT (no citric acid) | PVA-Alg-NaMT (no citric acid) | ||
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# Prepare 210 mL of saturated citric acid with 4% calcium chloride | # Prepare 210 mL of saturated citric acid with 4% calcium chloride | ||
## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water | ## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water | ||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette | |||
## Heat solution to 40°C while stirring for 30 min | ## Heat solution to 40°C while stirring for 30 min | ||
## Allow solution to cool to room temp while stirring for another 20 min | ## Allow solution to cool to room temp while stirring for another 20 min | ||
#Leave beads in solution, not stirring, overnight | #Leave beads in solution, not stirring, overnight |
Revision as of 08:45, 25 February 2015
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February 27, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT (no citric acid)
MeasurementsNotes |