User:Madeleine Y. Bee/Notebook/CHEM-582 Experimental Chemistry II/2015/02/27: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
Line 12: | Line 12: | ||
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.> | Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.> | ||
<br.> | <br.> | ||
PVA-Alg-NaMT | PVA-Alg-NaMT | ||
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | # Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) | ||
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate) | #* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate) | ||
Line 19: | Line 19: | ||
# Cool dissolved and mixed solution to 35°C | # Cool dissolved and mixed solution to 35°C | ||
# Prepare 210 mL of saturated citric acid with 4% calcium chloride | # Prepare 210 mL of saturated citric acid with 4% calcium chloride | ||
## Add 32 g citric acid | |||
## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water | ## Add '''8.4 g (4%) calcium chloride''' to 210 mL of water | ||
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette | # Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette |
Revision as of 09:14, 25 February 2015
PVA-Alg Bead Synthesis | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
February 27, 2015
ProcedureProcedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT
MeasurementsNotes |