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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PVA-Alg Bead Synthesis</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==, 2015==
==March 4, 2015==
*
*Repeat synthesis, shorten time in sodium sulfate and lengthen time in 10% citric acid solution


===Procedure===
===Procedure===
 
Procedure adapted from [http://onlinelibrary.wiley.com/doi/10.1002/bit.260390411/abstract | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)] and [http://www.sciencedirect.com/science/article/pii/S1369703X10000768 Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix]<br.>
<br.>
PVA-Alg-NaMT
# Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
#* '''1 g of PVA''' and '''0.16 g of sodium alginate''' in '''7 mL of water''' (12.5% PVA and ~0.02% alginate)
#** Add alginate '''while stirring''' PVA-water solution
#* '''~0.1 g of NaMT''' should be added to this mixture where applicable
# Cool dissolved and mixed solution to 35°C
# Prepare 210 mL of citric acid, calcium chloride solution
## Add '''12-15 g (5-7%) citric acid'''
## Add '''8.4 g (4%) calcium chloride'''
## Heat solution to 40°C while stirring
# Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
#Allow solution to cool to room temp while stirring for another 20 min
# Stir in '''24 g''' (10%) citric acid solution for 30 min
# Stir in '''14.9 g''' (0.5 M) sodium sulfate solution for 10 min
# Rinse with water to remove excess reactants
# Allow beads to dry in teflon plate overnight


====Measurements====
====Measurements====
 
*PVA: 1.06 g
*Alg: 0.16 g
*NaMT: 0.1 g
*citric acid: 12.09 g
*CaCl2: 8.42 g
*citric acid: 24.92 g
*sodium sulfate: 14.95 g


====Notes====
====Notes====

Latest revision as of 00:48, 27 September 2017

PVA-Alg Bead Synthesis Main project page
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March 4, 2015

  • Repeat synthesis, shorten time in sodium sulfate and lengthen time in 10% citric acid solution

Procedure

Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) and Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix<br.> <br.> PVA-Alg-NaMT

  1. Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C)
    • 1 g of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate)
      • Add alginate while stirring PVA-water solution
    • ~0.1 g of NaMT should be added to this mixture where applicable
  2. Cool dissolved and mixed solution to 35°C
  3. Prepare 210 mL of citric acid, calcium chloride solution
    1. Add 12-15 g (5-7%) citric acid
    2. Add 8.4 g (4%) calcium chloride
    3. Heat solution to 40°C while stirring
  4. Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette
  5. Allow solution to cool to room temp while stirring for another 20 min
  6. Stir in 24 g (10%) citric acid solution for 30 min
  7. Stir in 14.9 g (0.5 M) sodium sulfate solution for 10 min
  8. Rinse with water to remove excess reactants
  9. Allow beads to dry in teflon plate overnight

Measurements

  • PVA: 1.06 g
  • Alg: 0.16 g
  • NaMT: 0.1 g
  • citric acid: 12.09 g
  • CaCl2: 8.42 g
  • citric acid: 24.92 g
  • sodium sulfate: 14.95 g

Notes


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