User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26: Difference between revisions
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== | ==February 26, 2013== | ||
* | ===Hybridization of molecular beacon and complementary strand at varying concentrations=== | ||
====MB:CS (MB at 500pM)==== | |||
(7.3x10^-6M)V=(3x10^-6)(10μL)<br.> | |||
4.1μL MB in 5.9μL buffer | |||
<br.> | |||
(2.8x10^-5)V=(3x10^-6M)(10μL)<br.> | |||
1.1μL CS in 8.9μL buffer | |||
<br.> | |||
(3x10^-6)V=(500x10^-9)(50μL)<br.> | |||
8.33μL in 41.67μL | |||
<br.> | |||
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS | |||
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS | |||
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS | |||
<br.> | |||
====MB:CS (CS at 500pM)==== | |||
(7.3x10^-6)V=(5x10^-6)(5μL) | |||
3.42μL MB in 1.57μL buffer | |||
<br.> | |||
0.36μL CS in 9.64μL buffer | |||
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS | |||
*5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS | |||
*10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS | |||
*50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS | |||
<br.> | |||
=====Make 1nm, 500pM, and 100pM samples for each ratio===== | |||
1:1 | |||
*50nM: (500nM)V=(50nM)(50μL) | |||
5μL 500nM solution in 45μL buffer | |||
*1nM: (50nM)V=(1nM)(50μL) | |||
1μL 50nM solution in 49μL buffer | |||
*500pM (0.5nM): (1nM)V=(0.5nM)(50μL) | |||
25μL 1nM solution in 25μL buffer | |||
*100pM (0.1nM): (0.5nM)V=(0.1nM)(50μL) | |||
10μL 0.5nM solution in 40μL buffer | |||
1:5, 1:10, 5:1, 10:1, and 50:1 calculations done in the same way | |||
Revision as of 05:34, 18 March 2013
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February 26, 2013Hybridization of molecular beacon and complementary strand at varying concentrationsMB:CS (MB at 500pM)(7.3x10^-6M)V=(3x10^-6)(10μL)<br.> 4.1μL MB in 5.9μL buffer <br.> (2.8x10^-5)V=(3x10^-6M)(10μL)<br.> 1.1μL CS in 8.9μL buffer <br.> (3x10^-6)V=(500x10^-9)(50μL)<br.> 8.33μL in 41.67μL <br.>
<br.> MB:CS (CS at 500pM)(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer <br.> 0.36μL CS in 9.64μL buffer
<br.> Make 1nm, 500pM, and 100pM samples for each ratio1:1
5μL 500nM solution in 45μL buffer
1μL 50nM solution in 49μL buffer
25μL 1nM solution in 25μL buffer
10μL 0.5nM solution in 40μL buffer 1:5, 1:10, 5:1, 10:1, and 50:1 calculations done in the same way
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