User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26: Difference between revisions
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== | ==February 26, 2013== | ||
* | * Hybridization of molecular beacon and complementary strand at varying concentrations | ||
===MB:CS (MB at 500pM)=== | |||
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS | |||
(7.3x10^-6M)V=(3x10^-6)(10μL) | |||
4.1μL MB in 5.9μL buffer | |||
<br.> | |||
(2.8x10^-5)V=(3x10^-6M)(10μL) | |||
1.1μL CS in 8.9μL buffer | |||
<br.> | |||
(3x10^-6)V=(500x10^-9)(50μL) | |||
8.33μL MB in 41.67μL buffer | |||
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS | |||
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS | |||
<br.> | |||
===MB:CS (CS at 500pM)=== | |||
Revision as of 18:20, 17 March 2013
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February 26, 2013
MB:CS (MB at 500pM)
(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer <br.> (2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer <br.> (3x10^-6)V=(500x10^-9)(50μL) 8.33μL MB in 41.67μL buffer
<br.> MB:CS (CS at 500pM) | |
