User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26

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==Entry title==
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==March 26, 2013==
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* Insert content here...
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=====Dilutions of AuNP, MB, and thiol DNA Solution=====
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Goal: dilute solution to 1-0.5nM AuNP
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(9.7nM)V=(4.85nM)(100μL)
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V= 50μL 9.7nM AuNP in 50μL
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======serial dilutions done with 50mM HEPES buffer:======
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4.85nM
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2.425nM
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1.2125nM
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0.61nM
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0.303nM
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======FCS Data======
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*0.3nM
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Τ=0.00018 ms
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N= 0.41
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*0.61nM
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Τ=184.456 ms
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N= 620
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*0.3nM (trial 2)
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Τ=0.2046 ms
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N= 12
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*o.61nM (trial 2)
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Τ=0
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N=0
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======Prepare Smaller Intervals of Diluted Samples:======
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(2.425nM)V=(0.5nM)(50μL)
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        V=10.3μL in 39.7μL HEPES buffer
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0.4 nM → 8.2μL in 41.75μL
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0.3 nM → 6.2μL in 43.8μL
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0.2 nM → 4.1μL in 45.95μL
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Revision as of 11:33, 2 April 2013

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March 26, 2013

Dilutions of AuNP, MB, and thiol DNA Solution

Goal: dilute solution to 1-0.5nM AuNP (9.7nM)V=(4.85nM)(100μL) V= 50μL 9.7nM AuNP in 50μL

serial dilutions done with 50mM HEPES buffer:

4.85nM 2.425nM 1.2125nM 0.61nM 0.303nM

FCS Data
  • 0.3nM

Τ=0.00018 ms N= 0.41

  • 0.61nM

Τ=184.456 ms N= 620

  • 0.3nM (trial 2)

Τ=0.2046 ms N= 12

  • o.61nM (trial 2)

Τ=0 N=0

Prepare Smaller Intervals of Diluted Samples:

(2.425nM)V=(0.5nM)(50μL)

        V=10.3μL in 39.7μL HEPES buffer

0.4 nM → 8.2μL in 41.75μL 0.3 nM → 6.2μL in 43.8μL 0.2 nM → 4.1μL in 45.95μL




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