User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/16: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==April 16, 2013==
==April 16, 2013==
=====Reconstituting DNA and Supernatants=====
10μL of water used to reconstitute each sample of DNA and each of the four supernatants


=====Gel Electrophoresis=====
Procedure from Allison Alix [[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/16| 2013/04/16]]


=====Calculations for thiol DNA and ThT solution=====
*AuNP starting concentration: 19.4nM
*DNA starting concentration: 53.1μM
DNA required starting concentration: 5.82μM <br.>
(53.1μM)V=(5.82μM)(500μL)<br.>
V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.>
*thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM<br.>
<br.>
Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
*DNA concentration: 2.91μM
*ThT concentration: 2.5μM
<br.>
Procedure continued on April 23, 2013<br.>
<br.>


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 09:15, 24 April 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

April 16, 2013

Reconstituting DNA and Supernatants

10μL of water used to reconstitute each sample of DNA and each of the four supernatants

Gel Electrophoresis

Procedure from Allison Alix [2013/04/16]

Calculations for thiol DNA and ThT solution
  • AuNP starting concentration: 19.4nM
  • DNA starting concentration: 53.1μM

DNA required starting concentration: 5.82μM <br.> (53.1μM)V=(5.82μM)(500μL)<br.> V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.>

  • thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM<br.>

<br.> Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT

  • DNA concentration: 2.91μM
  • ThT concentration: 2.5μM

<br.> Procedure continued on April 23, 2013<br.> <br.>


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/04/16&oldid=693925"