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| |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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| ==May 27, 2013==
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| ===Preparing Silica-Coated AuNP===
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| from [[Gold and Silica-Coated Gold Nanparticles as Thermographic Labels for DNA Detection|http://pubs.acs.org/doi/full/10.1021/ac0600555]]
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| ====Chemicals Needed====
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| *TES (tetraethyl orthosilicate)
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| *NH<sub>4</sub>OH
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| *2-propanol
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| *MHA
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| ====Procedure====
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| *Add TES (tetraethyl orthosilicate) to 10nm diameter 9.4nM AuNPs, NH<sub>4</sub>OH used as a catalyst, reaction carried out in 2-propanol, volumes were varied:
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| #1mL 9.4nM, 10nm AuNP/no stabilizer/10μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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| #1mL 9.4nM, 10nm AuNP/no stabilizer/20μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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| #1mL 9.4nM, 10nm AuNP/no stabilizer/40μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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| #1mL 9.4nM, 10nm AuNP/MHA/5μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol <br.>
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| **Attach MHA (for stability):
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| ***Add 5mL of 10mM MHA to 5mL of AuNPs, stir overnight
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| *Stir solutions for one hour
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| *Centrifuge solutions at 4000 rpm for one hour
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| *Re-suspend particles in water up to a final concentration of 5nM
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| ====Calculations====
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| __NOTOC__
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