User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/05/27

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

May 27, 2013

Preparing Silica-Coated AuNP

from http://pubs.acs.org/doi/full/10.1021/ac0600555

Chemicals Needed

  • TES (tetraethyl orthosilicate)
  • NH4OH
  • 2-propanol
  • MHA

Procedure

  • Add TES (tetraethyl orthosilicate) to 10nm diameter 9.4nM AuNPs, NH4OH used as a catalyst, reaction carried out in 2-propanol, volumes were varied:
  1. 1mL 9.4nM, 10nm AuNP/no stabilizer/10μL TES/0.125mL NH4OH/5mL propanol
  2. 1mL 9.4nM, 10nm AuNP/no stabilizer/20μL TES/0.125mL NH4OH/5mL propanol
  3. 1mL 9.4nM, 10nm AuNP/no stabilizer/40μL TES/0.125mL NH4OH/5mL propanol
  4. 1mL 9.4nM, 10nm AuNP/MHA/5μL TES/0.125mL NH4OH/5mL propanol <br.>
    • Attach MHA (for stability):
      • Add 5mL of 10mM MHA to 5mL of AuNPs, stir overnight
  • Stir solutions for one hour
  • Centrifuge solutions at 4000 rpm for one hour
  • Re-suspend particles in water up to a final concentration of 5nM

Calculations


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/27&oldid=699440"