User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/10

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(June 10, 2013)
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*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed<br.>
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed<br.>
**MHA attachment performed, silica-coating reaction to be performed tomorrow
**MHA attachment performed, silica-coating reaction to be performed tomorrow
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+
===Buffer Preparation and Calculations===
 +
Sodium phosphate buffer, pH 9, to be used for the modification of HS-DNA and AuNPs@SiO<sub>2</sub> procedure.<br.>
 +
monosodium phosphate- MW: 119.98g/mol<br.>
 +
disodium phosphate- MW: 141.96g/mol, pKA: 6.82<br.>
 +
buffer- pH 9,concentration 10mM<br.>
 +
<br.>
 +
9=6.82+log([base]/[acid])<br.>
 +
151.36=(10-[base])/([acid])<br.>
 +
[acid](151.36+1)=10<br.>
 +
[acid]=0.066mM<br.>
 +
[base]=9.935mM<br.>
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<br.>
 +
Use 0.0094g NaH<sub>2</sub>PO<sub>4</sub> and 1.19188g Na<sub>2</sub>HPO<sub>4</sub> in 1L water.<br.>

Revision as of 11:32, 10 June 2013

Silica-coating Reaction Main project page
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June 10, 2013

  • Silica-coating procedure from 05/24/2013 performed
    • MHA attachment performed, silica-coating reaction to be performed tomorrow

Buffer Preparation and Calculations

Sodium phosphate buffer, pH 9, to be used for the modification of HS-DNA and AuNPs@SiO2 procedure.
monosodium phosphate- MW: 119.98g/mol
disodium phosphate- MW: 141.96g/mol, pKA: 6.82
buffer- pH 9,concentration 10mM

9=6.82+log([base]/[acid])
151.36=(10-[base])/([acid])
[acid](151.36+1)=10
[acid]=0.066mM
[base]=9.935mM

Use 0.0094g NaH2PO4 and 1.19188g Na2HPO4 in 1L water.



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