User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/10: Difference between revisions
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*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed<br.> | *Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed<br.> | ||
**MHA attachment performed, silica-coating reaction to be performed tomorrow | **MHA attachment performed, silica-coating reaction to be performed tomorrow | ||
===Buffer Preparation and Calculations=== | |||
Sodium phosphate buffer, pH 9, to be used for the modification of HS-DNA and AuNPs@SiO<sub>2</sub> procedure.<br.> | |||
monosodium phosphate- MW: 119.98g/mol<br.> | |||
disodium phosphate- MW: 141.96g/mol, pKA: 6.82<br.> | |||
buffer- pH 9,concentration 10mM<br.> | |||
<br.> | |||
9=6.82+log([base]/[acid])<br.> | |||
151.36=(10-[base])/([acid])<br.> | |||
[acid](151.36+1)=10<br.> | |||
[acid]=0.066mM<br.> | |||
[base]=9.935mM<br.> | |||
<br.> | |||
Use 0.0094g NaH<sub>2</sub>PO<sub>4</sub> and 1.19188g Na<sub>2</sub>HPO<sub>4</sub> in 1L water.<br.> | |||
Revision as of 08:32, 10 June 2013
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June 10, 2013
Buffer Preparation and CalculationsSodium phosphate buffer, pH 9, to be used for the modification of HS-DNA and AuNPs@SiO2 procedure.<br.> monosodium phosphate- MW: 119.98g/mol<br.> disodium phosphate- MW: 141.96g/mol, pKA: 6.82<br.> buffer- pH 9,concentration 10mM<br.> <br.> 9=6.82+log([base]/[acid])<br.> 151.36=(10-[base])/([acid])<br.> [acid](151.36+1)=10<br.> [acid]=0.066mM<br.> [base]=9.935mM<br.> <br.> Use 0.0094g NaH2PO4 and 1.19188g Na2HPO4 in 1L water.<br.>
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