User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/10: Difference between revisions
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====Reactions==== | ====Reactions==== | ||
====Chemicals Needed and Safety==== | ====Chemicals Needed and Safety==== | ||
*Ethanol | |||
*15μL, 1mM APS ((3-aminopropyl)trimethoxysilane) | |||
*1mg SSMCC (sulfosuccinimidyl-4-N-maleimidomethyl cyclohexane-1-carboxylate) | |||
**Must be stored at -20°C | |||
**Moisture sensitive, store in desiccant | |||
**[http://www.piercenet.com/instructions/2160581.pdf Manufacturer Instructions] | |||
*Potassium phosphate buffer, pH 7.4 | |||
*Sodium phosphate buffer, pH 9 | |||
*150μL Silica-coated AuNPs | |||
*50μL of 20μM HS-DNA | |||
====Procedure==== | ====Procedure==== | ||
*Combine 150μL AuNPs@SiO<sub>2</sub> (prepared 06/10/2013), 15μL APS and 235μL ethanol | *Combine 150μL AuNPs@SiO<sub>2</sub> (prepared 06/10/2013), 15μL APS and 235μL ethanol |
Revision as of 10:02, 10 June 2013
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June 10, 2013
Buffer Preparation and CalculationsSodium phosphate buffer, pH 9, to be used for the modification of HS-DNA and AuNPs@SiO2 procedure.<br.> monosodium phosphate- MW: 119.98g/mol<br.> disodium phosphate- MW: 141.96g/mol, pKA: 6.82<br.> buffer- pH 9,concentration 10mM<br.> <br.> 9=6.82+log([base]/[acid])<br.> 151.36=(10-[base])/([acid])<br.> [acid](151.36+1)=10<br.> [acid]=0.066mM<br.> [base]=9.935mM<br.> <br.> Use 0.0094g NaH2PO4 and 1.19188g Na2HPO4 in 1L water.<br.> New: Modifying Silica-Coated AuNPs with HS-DNA ProcedureReactionsChemicals Needed and Safety
Procedure
Calculations |