User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Spectra</span> | ||
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==June 11, 2013== | ==June 11, 2013== | ||
*Absorbance spectra taken of samples prepared yesterday, 06/10/2013 | *Absorbance spectra taken of samples prepared yesterday, 06/10/2013 | ||
*Fluormeter computer was down, hopefully spectra will be taken | *Fluormeter computer was down, hopefully spectra will be taken as soon as possible | ||
*MHA silica-coating procedure completed, spectra to be taken | *MHA silica-coating procedure completed, spectra to be taken as soon as possible | ||
===Absorbance Data=== | ===Absorbance Data=== | ||
DNA at varied concentrations and ThT at 2.5μM | ====DNA at varied concentrations and ThT at 2.5μM==== | ||
The larger peaks around 260nm are due to the DNA, while the smaller peaks around 430nm are due to the ThT.<br.> | The larger peaks around 260nm are due to the DNA, while the smaller peaks around 430nm are due to the ThT. These samples will be excited at 430nm to observe fluorescence, and a calibration curve will be constructed.<br.> | ||
[[Image:Abs_data_2.5uM_ThT_and_varied_DNA.png]] | [[Image:Abs_data_2.5uM_ThT_and_varied_DNA.png]] | ||
<br.> | <br.> | ||
<br.> | <br.> | ||
Silica-coated AuNP comparison | ====Silica-coated AuNP comparison==== | ||
There was no absorbance observed in any of the samples. The silica-coated particles may have been too concentrated; they will be diluted and run again tomorrow. The original particles may have been clumped and since the concentration is small, they may not have been detected. They will be sonicated, to separate the potentially clumped particles, and run again tomorrow with some samples of varying concentrations of the original nanoparticles.<br.> | There was no absorbance observed in any of the samples. The silica-coated particles may have been too concentrated; they will be diluted and run again tomorrow. The original particles may have been clumped and since the concentration is small, they may not have been detected. They will be sonicated, to separate the potentially clumped particles, and run again tomorrow with some samples of varying concentrations of the original nanoparticles.<br.> | ||
[[Image:Abs_data_AuNPs_and_AuNPs@SiO2_06112013.png]] | [[Image:Abs_data_AuNPs_and_AuNPs@SiO2_06112013.png]] | ||
Revision as of 05:49, 14 June 2013
 Absorbance Spectra
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June 11, 2013
Absorbance DataDNA at varied concentrations and ThT at 2.5μMThe larger peaks around 260nm are due to the DNA, while the smaller peaks around 430nm are due to the ThT. These samples will be excited at 430nm to observe fluorescence, and a calibration curve will be constructed.<br.>
Silica-coated AuNP comparisonThere was no absorbance observed in any of the samples. The silica-coated particles may have been too concentrated; they will be diluted and run again tomorrow. The original particles may have been clumped and since the concentration is small, they may not have been detected. They will be sonicated, to separate the potentially clumped particles, and run again tomorrow with some samples of varying concentrations of the original nanoparticles.<br.>
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