User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/19

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(Autocreate 2013/06/19 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
Current revision (14:44, 20 June 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Prep</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==June 19, 2013==
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* Insert content here...
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*Absorbance and fluorescence measurements taken. Inconclusive results; new samples to be made and run again.
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===Procedure Prep: MHA===
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*Calculations from 06/10/13
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**0.0144g MHA in 5mL PBS to make 10mM MHA
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**Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
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**Stir overnight
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===Calculations: RCF to RPM Conversion===
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RCF=0.00001118rN<sup>2</sup><br.>
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7700g=0.00001118(8.35cm)N<sup>2</sup><br.>
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N=9081.99 RPM

Current revision

Prep Main project page
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June 19, 2013

  • Absorbance and fluorescence measurements taken. Inconclusive results; new samples to be made and run again.

Procedure Prep: MHA

  • Calculations from 06/10/13
    • 0.0144g MHA in 5mL PBS to make 10mM MHA
    • Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
    • Stir overnight

Calculations: RCF to RPM Conversion

RCF=0.00001118rN2
7700g=0.00001118(8.35cm)N2
N=9081.99 RPM



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