User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/19: Difference between revisions

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==Entry title==
==June 19, 2013==
* Insert content here...
===Procedure Prep: MHA===
*Calculations from 06/10/13
**0.0144g MHA in 5mL PBS to make 10mM MHA
**Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
**Stir overnight
===Calculations: RCF to RPM Conversion===
RCF=0.00001118rN<sup>2</sup><br.>
7700g=0.00001118(8.35cm)N<sup>2</sup><br.>
N=9081.99 RPM




Revision as of 09:04, 19 June 2013

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June 19, 2013

Procedure Prep: MHA

  • Calculations from 06/10/13
    • 0.0144g MHA in 5mL PBS to make 10mM MHA
    • Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
    • Stir overnight

Calculations: RCF to RPM Conversion

RCF=0.00001118rN2<br.> 7700g=0.00001118(8.35cm)N2<br.> N=9081.99 RPM



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