User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/19

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==Entry title==
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==June 19, 2013==
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* Insert content here...
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===Procedure Prep: MHA===
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*Calculations from 06/10/13
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**0.0144g MHA in 5mL PBS to make 10mM MHA
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**Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
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**Stir overnight
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===Calculations: RCF to RPM Conversion===
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RCF=0.00001118rN<sup>2</sup><br.>
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7700g=0.00001118(8.35cm)N<sup>2</sup><br.>
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N=9081.99 RPM

Revision as of 12:04, 19 June 2013

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June 19, 2013

Procedure Prep: MHA

  • Calculations from 06/10/13
    • 0.0144g MHA in 5mL PBS to make 10mM MHA
    • Add 5mL 9.4nM, 10nm AuNPs (diluted in PBS)
    • Stir overnight

Calculations: RCF to RPM Conversion

RCF=0.00001118rN2
7700g=0.00001118(8.35cm)N2
N=9081.99 RPM



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