User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20: Difference between revisions
From OpenWetWare
(Autocreate 2013/06/20 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence) |
(fix raw html notebook nav) |
||
(3 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Spectra and Calibration Graph</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==June 20, 2013== | ||
* | ===Sample Prep: New DNA/ThT Solutions=== | ||
*Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples | |||
*Run absorbance and fluorescence | |||
*Create calibration graph | |||
====Fluorescence Data==== | |||
[[Image:Fluor_data_varying_DNA%2C_2.5_ThT.png]] | |||
====Calibration Graph==== | |||
[[Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png]] | |||
===Silica-Coating Procedure=== | |||
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed | |||
**1mL AuNPs/20uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | |||
**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | |||
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | |||
*Each of the three solutions were centrifuged at 4000rpm for 1 hour | |||
*Each of the three solutions were re-dispersed in 140uL PBS buffer | |||
====Absorbance Spectra==== | |||
[[Image:Abs_data_silica-coating_0620.png]]<br.> | |||
Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra. | |||
Latest revision as of 22:46, 26 September 2017
Absorbance Spectra and Calibration Graph | Main project page Previous entry Next entry |
June 20, 2013Sample Prep: New DNA/ThT Solutions
Fluorescence DataCalibration GraphSilica-Coating Procedure
Absorbance Spectra<br.> Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.
|