User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Spectra and Calibration Graph</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 11: | Line 11: | ||
*Run absorbance and fluorescence | *Run absorbance and fluorescence | ||
*Create calibration graph | *Create calibration graph | ||
====Fluorescence Data==== | |||
[[Image:Fluor_data_varying_DNA%2C_2.5_ThT.png]] | |||
====Calibration Graph==== | |||
[[Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png]] | |||
===Silica-Coating Procedure=== | ===Silica-Coating Procedure=== | ||
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed | *Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed | ||
Line 16: | Line 20: | ||
**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | **1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | ||
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | **1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | ||
* | *Each of the three solutions were centrifuged at 4000rpm for 1 hour | ||
*Each of the three solutions were re-dispersed in 140uL PBS buffer | |||
====Absorbance Spectra==== | |||
[[Image:Abs_data_silica-coating_0620.png]]<br.> | |||
Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra. | |||
Latest revision as of 22:46, 26 September 2017
Absorbance Spectra and Calibration Graph | Main project page Previous entry Next entry |
June 20, 2013Sample Prep: New DNA/ThT Solutions
Fluorescence DataCalibration GraphSilica-Coating Procedure
Absorbance Spectra<br.> Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.
|