User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20

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Absorbance Spectra and Calibration Graph Main project page
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June 20, 2013

Sample Prep: New DNA/ThT Solutions

  • Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
  • Run absorbance and fluorescence
  • Create calibration graph

Fluorescence Data

Image:Fluor_data_varying_DNA,_2.5_ThT.png

Calibration Graph

Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png

Silica-Coating Procedure

  • Silica-coating procedure from 05/24/2013 performed
    • 1mL AuNPs/20uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs/40uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs and MHA solution/5uL TES/0.125mL NH4OH/5mL isopropanol
  • Each of the three solutions were centrifuged at 4000rpm for 1 hour
  • Each of the three solutions were re-dispersed in 140uL PBS buffer

Absorbance Spectra

Image:Abs_data_silica-coating_0620.png
Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.



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