User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20

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(June 20, 2013)
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*Run absorbance and fluorescence
*Run absorbance and fluorescence
*Create calibration graph
*Create calibration graph
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====Fluorescence Data====
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[[Image:Fluor_data_varying_DNA%2C_2.5_ThT.png]]
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====Calibration Graph====
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[[Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png]]
===Silica-Coating Procedure===
===Silica-Coating Procedure===
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed
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**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
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*Take absorbance spectra of original AuNPs and three silica-coated AuNP solutions
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*Each of the three solutions were centrifuged at 4000rpm for 1 hour
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*Each of the three solutions were re-dispersed in 140uL PBS buffer
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====Absorbance Spectra====
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[[Image:Abs_data_silica-coating_0620.png]]<br.>
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Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.

Revision as of 15:32, 20 June 2013

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June 20, 2013

Sample Prep: New DNA/ThT Solutions

  • Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
  • Run absorbance and fluorescence
  • Create calibration graph

Fluorescence Data

Image:Fluor_data_varying_DNA,_2.5_ThT.png

Calibration Graph

Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png

Silica-Coating Procedure

  • Silica-coating procedure from 05/24/2013 performed
    • 1mL AuNPs/20uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs/40uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs and MHA solution/5uL TES/0.125mL NH4OH/5mL isopropanol
  • Each of the three solutions were centrifuged at 4000rpm for 1 hour
  • Each of the three solutions were re-dispersed in 140uL PBS buffer

Absorbance Spectra

Image:Abs_data_silica-coating_0620.png
Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.



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