User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20

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==Entry title==
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==June 20, 2013==
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===Sample Prep: New DNA/ThT Solutions===
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*Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
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*Run absorbance and fluorescence
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*Create calibration graph
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===Silica-Coating Procedure===
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*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed
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**1mL AuNPs/20uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
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**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
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**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
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*Take absorbance spectra of original AuNPs and three silica-coated AuNP solutions

Revision as of 10:21, 20 June 2013

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June 20, 2013

Sample Prep: New DNA/ThT Solutions

  • Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
  • Run absorbance and fluorescence
  • Create calibration graph

Silica-Coating Procedure

  • Silica-coating procedure from 05/24/2013 performed
    • 1mL AuNPs/20uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs/40uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs and MHA solution/5uL TES/0.125mL NH4OH/5mL isopropanol
  • Take absorbance spectra of original AuNPs and three silica-coated AuNP solutions



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