User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Absorbance Spectra and Calibration Graph <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

June 20, 2013

Sample Prep: New DNA/ThT Solutions

  • Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
  • Run absorbance and fluorescence
  • Create calibration graph

Fluorescence Data

Calibration Graph

Silica-Coating Procedure

  • Silica-coating procedure from 05/24/2013 performed
    • 1mL AuNPs/20uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs/40uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs and MHA solution/5uL TES/0.125mL NH4OH/5mL isopropanol
  • Each of the three solutions were centrifuged at 4000rpm for 1 hour
  • Each of the three solutions were re-dispersed in 140uL PBS buffer

Absorbance Spectra

<br.> Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/20&oldid=704282"