User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/21

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June 21, 2013

Notes from Meeting

  • Check pH of sodium citrate buffer (color change?)
    • Read literature for salt effects on AuNPs
  • Make lower concentration dilutions of DNA/ThT solutions by serial dilutions
    • Plot the ratio rather than the DNA concentration
  • Use the same wavelength for the calibration curve; take zero point (ThT) out of the calibration curve
  • Tuesday: run MB samples on the FCS with Dr Miller
  • Dilute 10x AuNPs@SiO2
    • Read literature for alternative detection methods
  • Endnote

Absorbance Data


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