User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/26: Difference between revisions
From OpenWetWare
(Autocreate 2013/06/26 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==June 26, 2013== | ||
* | ===Notes from Literature=== | ||
Looking for information on silica-coating thickness for DNA attachment procedure. | |||
*From: http://pubs.acs.org/doi/pdf/10.1021/nn401775e | |||
**References 8-13, 15, 26 | |||
**"Gold nanoparticles between 10 and 20 nm in diameter are important because they are too small to scatter light significantly, but exhibit a well-defined LSPR and an enhanced electromagnetic near-field. It is established that fluorescence of molecules in close proximity to these particles is quenched." | |||
**"...almost complete (>90%) quenching at short separations to negligible electromagnetic coupling at a distance of 43 nm" | |||
**"...hydrolysis of the reactive NHS moiety on the dye is much slower in ethanol than in water." (For attachment procedure) | |||
**"...attachment was more efficient for particles with a thicker silica shell due to the higher surface area and the increased number of binding sites available per particle." | |||
**"For average dye separations above 15 nm (less than 30 dyes per particle), only the effect of the dye's surface confinement is seen resulting in a small (<5%) decrease in fluorescence lifetime and in a 20% decrease in steady-state fluorescence relative to the fluorescence of the free dye. A very pronounced decrease in both fluorescence intensity and lifetime is observed for dye separations below 10 nm (more than 75 dyes per particle). For the highest dye loading, the steady-state fluorescence decreases by more than 90% and the fluorescence lifetime is reduced to 0.5 ns compared to 4.0 ns for the free dye in solution." | |||
*Solvents: ethanol for coated spheres, 2-propanol for uncoated | |||
===FCS Measurements=== | |||
Revision as of 10:08, 26 June 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
June 26, 2013Notes from LiteratureLooking for information on silica-coating thickness for DNA attachment procedure.
FCS Measurements |