User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/26

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==Entry title==
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==June 26, 2013==
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* Insert content here...
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===Notes from Literature===
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Looking for information on silica-coating thickness for DNA attachment procedure.
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*From: http://pubs.acs.org/doi/pdf/10.1021/nn401775e
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**References 8-13, 15, 26
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**"Gold nanoparticles between 10 and 20 nm in diameter are important because they are too small to scatter light significantly, but exhibit a well-defined LSPR and an enhanced electromagnetic near-field. It is established that fluorescence of molecules in close proximity to these particles is quenched."
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**"...almost complete (>90%) quenching at short separations to negligible electromagnetic coupling at a distance of 43 nm"
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**"...hydrolysis of the reactive NHS moiety on the dye is much slower in ethanol than in water." (For attachment procedure)
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**"...attachment was more efficient for particles with a thicker silica shell due to the higher surface area and the increased number of binding sites available per particle."
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**"For average dye separations above 15 nm (less than 30 dyes per particle), only the effect of the dye's surface confinement is seen resulting in a small (<5%) decrease in fluorescence lifetime and in a 20% decrease in steady-state fluorescence relative to the fluorescence of the free dye. A very pronounced decrease in both fluorescence intensity and lifetime is observed for dye separations below 10 nm (more than 75 dyes per particle). For the highest dye loading, the steady-state fluorescence decreases by more than 90% and the fluorescence lifetime is reduced to 0.5 ns compared to 4.0 ns for the free dye in solution."
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*Solvents: ethanol for coated spheres, 2-propanol for uncoated
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===FCS Measurements===
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Revision as of 13:08, 26 June 2013

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June 26, 2013

Notes from Literature

Looking for information on silica-coating thickness for DNA attachment procedure.

  • From: http://pubs.acs.org/doi/pdf/10.1021/nn401775e
    • References 8-13, 15, 26
    • "Gold nanoparticles between 10 and 20 nm in diameter are important because they are too small to scatter light significantly, but exhibit a well-defined LSPR and an enhanced electromagnetic near-field. It is established that fluorescence of molecules in close proximity to these particles is quenched."
    • "...almost complete (>90%) quenching at short separations to negligible electromagnetic coupling at a distance of 43 nm"
    • "...hydrolysis of the reactive NHS moiety on the dye is much slower in ethanol than in water." (For attachment procedure)
    • "...attachment was more efficient for particles with a thicker silica shell due to the higher surface area and the increased number of binding sites available per particle."
    • "For average dye separations above 15 nm (less than 30 dyes per particle), only the effect of the dye's surface confinement is seen resulting in a small (<5%) decrease in fluorescence lifetime and in a 20% decrease in steady-state fluorescence relative to the fluorescence of the free dye. A very pronounced decrease in both fluorescence intensity and lifetime is observed for dye separations below 10 nm (more than 75 dyes per particle). For the highest dye loading, the steady-state fluorescence decreases by more than 90% and the fluorescence lifetime is reduced to 0.5 ns compared to 4.0 ns for the free dye in solution."
  • Solvents: ethanol for coated spheres, 2-propanol for uncoated

FCS Measurements


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