User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/26

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(FCS Measurements)
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*Solvents: ethanol for coated spheres, 2-propanol for uncoated
*Solvents: ethanol for coated spheres, 2-propanol for uncoated
===FCS Measurements===
===FCS Measurements===
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*Samples prepared:
 +
**(1) 100pM DNA/120pM MB
 +
**(2) 80pM DNA/96pM MB
 +
**(3) 60pM DNA/72pM MB
 +
**(4) 40pM DNA/48pM MB
 +
**(5) 20pM DNA/24pM MB
 +
**(6) 100pM Oligo D
 +
**(7) 80pM Oligo D
 +
**(8) 60pM Oligo D

Revision as of 12:43, 26 June 2013

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June 26, 2013

Notes from Literature

Looking for information on silica-coating thickness for DNA attachment procedure.

  • From: http://pubs.acs.org/doi/pdf/10.1021/nn401775e
    • References 8-13, 15, 26
    • "Gold nanoparticles between 10 and 20 nm in diameter are important because they are too small to scatter light significantly, but exhibit a well-defined LSPR and an enhanced electromagnetic near-field. It is established that fluorescence of molecules in close proximity to these particles is quenched."
    • "...almost complete (>90%) quenching at short separations to negligible electromagnetic coupling at a distance of 43 nm"
    • "...hydrolysis of the reactive NHS moiety on the dye is much slower in ethanol than in water." (For attachment procedure)
    • "...attachment was more efficient for particles with a thicker silica shell due to the higher surface area and the increased number of binding sites available per particle."
    • "For average dye separations above 15 nm (less than 30 dyes per particle), only the effect of the dye's surface confinement is seen resulting in a small (<5%) decrease in fluorescence lifetime and in a 20% decrease in steady-state fluorescence relative to the fluorescence of the free dye. A very pronounced decrease in both fluorescence intensity and lifetime is observed for dye separations below 10 nm (more than 75 dyes per particle). For the highest dye loading, the steady-state fluorescence decreases by more than 90% and the fluorescence lifetime is reduced to 0.5 ns compared to 4.0 ns for the free dye in solution."
  • Solvents: ethanol for coated spheres, 2-propanol for uncoated

FCS Measurements

  • Samples prepared:
    • (1) 100pM DNA/120pM MB
    • (2) 80pM DNA/96pM MB
    • (3) 60pM DNA/72pM MB
    • (4) 40pM DNA/48pM MB
    • (5) 20pM DNA/24pM MB
    • (6) 100pM Oligo D
    • (7) 80pM Oligo D
    • (8) 60pM Oligo D




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