(Difference between revisions)
Revision as of 12:55, 26 June 2013
| Reading and FCS Data
|| Main project page|
Previous entry Next entry
June 26, 2013
Notes from Literature
Looking for information on silica-coating thickness for DNA attachment procedure.
- From: Distance and Wavelength Dependent Quenching of Molecular Fluorescence by Au@SiO2 Core-Shell Nanoparticles
- References 8-13, 15, 26
- "Gold nanoparticles between 10 and 20 nm in diameter are important because they are too small to scatter light significantly[...]fluorescence of molecules in close proximity to these particles is quenched."
- "...almost complete (>90%) quenching at short separations to negligible electromagnetic coupling at a distance of 43 nm"
- "...hydrolysis of the reactive NHS moiety on the dye is much slower in ethanol than in water." (For attachment procedure)
- "...attachment was more efficient for particles with a thicker silica shell due to the higher surface area and the increased number of binding sites available per particle."
- "For average dye separations above 15 nm (less than 30 dyes per particle)[...]small (<5%) decrease in fluorescence lifetime and in a 20% decrease in steady-state fluorescence relative to the fluorescence of the free dye. A very pronounced decrease in both fluorescence intensity and lifetime is observed for dye separations below 10 nm (more than 75 dyes per particle). For the highest dye loading, the steady-state fluorescence decreases by more than 90% and the fluorescence lifetime is reduced to 0.5 ns compared to 4.0 ns for the free dye in solution."
- Solvents: ethanol for coated spheres, 2-propanol for uncoated
- Samples prepared to be added to data and calibration graphs from 06/25/2013:
- (1) 100pM DNA/120pM MB
- (2) 80pM DNA/96pM MB
- (3) 60pM DNA/72pM MB
- (4) 40pM DNA/48pM MB
- (5) 20pM DNA/24pM MB
- (6) 100pM Oligo D
- (7) 80pM Oligo D
- (8) 60pM Oligo D
DNA/ThT Sample Prep
- Another 700nM sample was made to complete the calibration curve for the DNA/ThT samples
- Fluorescence measurements taken