User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27: Difference between revisions
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== | ==June 27, 2013== | ||
* | ===DNA/ThT Samples for Calibration Graph=== | ||
*Remade samples at 500, 600, 700, 800, 900nM | |||
*Fluorescence measurements taken | |||
====Results==== | |||
[[Image:Fluor_data_DNA_ThT_compliation.png]]<br.> | |||
<br.> | |||
[[Image:Fluor_data_DNA_ThT_compliation_calibration.png]]<br.> | |||
<br.> | |||
=====Inconsistent Spectra===== | |||
[[Image:Fluor_data_600nM_DNA_comparison.png]]<br.> | |||
<br.> | |||
[[Image:Fluor_data_700nM_DNA_comparison.png]]<br.> | |||
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.> | |||
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===Notes=== | |||
We still cannot produce consistent spectra for concentrations about 500nM. This may be due to a limit of bonding: only a certain about of ThT molecules can bind to a give number of DNA molecules and that number has been reached, making higher concentrations irrelevant. | |||
Revision as of 08:00, 27 June 2013
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June 27, 2013DNA/ThT Samples for Calibration Graph
Results
Inconsistent Spectra
NotesWe still cannot produce consistent spectra for concentrations about 500nM. This may be due to a limit of bonding: only a certain about of ThT molecules can bind to a give number of DNA molecules and that number has been reached, making higher concentrations irrelevant.
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