User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27

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(Notes)
(June 27, 2013)
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
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===Notes===
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====Notes====
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
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===FCS Measurements===
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*Samples were prepared again for the Oligo D and DNA/MB
 +
**Oligo D concentrations: 20, 40, 60, 80, 100p<
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**
   
   

Revision as of 13:21, 27 June 2013

Fluorescence and FCS Data Main project page
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June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Image:Fluor_data_DNA_ThT_compliation.png

Image:Fluor_data_DNA_ThT_compliation_calibration.png

Inconsistent Spectra

Image:Fluor_data_600nM_DNA_comparison.png

Image:Fluor_data_700nM_DNA_comparison.png

Image:Fluor_data_800nM_DNA_comparison.png

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB
    • Oligo D concentrations: 20, 40, 60, 80, 100p<



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