User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27

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(June 27, 2013)
Current revision (16:31, 28 June 2013) (view source)
(FCS Measurements)
 
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===FCS Measurements===
===FCS Measurements===
*Samples were prepared again for the Oligo D and DNA/MB
*Samples were prepared again for the Oligo D and DNA/MB
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**Oligo D concentrations: 20, 40, 60, 80, 100p<
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Current revision

Fluorescence and FCS Data Main project page
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June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Image:Fluor_data_DNA_ThT_compliation.png

Image:Fluor_data_DNA_ThT_compliation_calibration.png

Inconsistent Spectra

Image:Fluor_data_600nM_DNA_comparison.png

Image:Fluor_data_700nM_DNA_comparison.png

Image:Fluor_data_800nM_DNA_comparison.png

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB




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