User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/28: Difference between revisions

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==June 28, 2013==
==June 28, 2013==
===Notes from Meeting===
===Notes from Meeting===
*
*Find averages and standard deviation of inconsistent DNA/ThT spectra for calibration curve
**Fit Gaussian to inconsistent spectra
*Check literature for AuNP@SiO2 shift and silica-shell radius calculations
*Label axes for FCS data
*FCS spectra for fluorescent beads in prezi
*FCS: increase time as concentration decreases
*Repeat old AuNP procedure with solutions from new procedure
===FCS Measurements===
*Prepare Samples
**10000x diluted green fluorescent beads
**100, 80, 60, 20pM  Oligo D
**10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples
====Results====
 




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June 28, 2013

Notes from Meeting

  • Find averages and standard deviation of inconsistent DNA/ThT spectra for calibration curve
    • Fit Gaussian to inconsistent spectra
  • Check literature for AuNP@SiO2 shift and silica-shell radius calculations
  • Label axes for FCS data
  • FCS spectra for fluorescent beads in prezi
  • FCS: increase time as concentration decreases
  • Repeat old AuNP procedure with solutions from new procedure

FCS Measurements

  • Prepare Samples
    • 10000x diluted green fluorescent beads
    • 100, 80, 60, 20pM Oligo D
    • 10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples

Results


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