User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/28

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==June 28, 2013==
==June 28, 2013==
===Notes from Meeting===
===Notes from Meeting===
-
*
+
*Find averages and standard deviation of inconsistent DNA/ThT spectra for calibration curve
 +
**Fit Gaussian to inconsistent spectra
 +
*Check literature for AuNP@SiO2 shift and silica-shell radius calculations
 +
*Label axes for FCS data
 +
*FCS spectra for fluorescent beads in prezi
 +
*FCS: increase time as concentration decreases
 +
*Repeat old AuNP procedure with solutions from new procedure
 +
===FCS Measurements===
 +
*Prepare Samples
 +
**10000x diluted green fluorescent beads
 +
**100, 80, 60, 20pM  Oligo D
 +
**10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples
 +
====Results====
 +
 

Revision as of 11:01, 28 June 2013

Meeting, Main project page
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June 28, 2013

Notes from Meeting

  • Find averages and standard deviation of inconsistent DNA/ThT spectra for calibration curve
    • Fit Gaussian to inconsistent spectra
  • Check literature for AuNP@SiO2 shift and silica-shell radius calculations
  • Label axes for FCS data
  • FCS spectra for fluorescent beads in prezi
  • FCS: increase time as concentration decreases
  • Repeat old AuNP procedure with solutions from new procedure

FCS Measurements

  • Prepare Samples
    • 10000x diluted green fluorescent beads
    • 100, 80, 60, 20pM Oligo D
    • 10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples

Results


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