User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/28

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(June 28, 2013)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Meeting,FCS</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Meeting and FCS Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-

Revision as of 16:08, 28 June 2013

Meeting and FCS Measurements Main project page
Previous entry      Next entry

June 28, 2013

Notes from Meeting

  • Find averages and standard deviation of inconsistent DNA/ThT spectra for calibration curve
    • Fit Gaussian for inconsistent spectra (use Igor Pro program)
  • Check literature for AuNP@SiO2 shift and silica-shell radius calculations
  • Label axes for FCS data
  • FCS spectra for fluorescent beads in prezi
  • FCS: increase time as concentration decreases
  • Repeat old AuNP procedure with solutions from new procedure

FCS Measurements

  • Prepare Samples
    • 10000x diluted green fluorescent beads
    • 100, 80, 60, 20pM Oligo D
    • 10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples

Results and Notes

Calibration graphs:
Image:FCS_data_2013_0628_calibration_graphs.PNG

  • Oligo D samples were run for 300s, the 60pM and 80pM samples are still inconsistent with the trend of the other concentrations
  • The first round of MB/DNA samples were run for 300s, results had lots of noise and inconsistencies
  • The second round of MB/DNA samples were run for 700s to eliminate noise, only the 25pM sample lied outside the trend of the other concentrations
  • Future work:
    • Always align laser with 10000x diluted green fluorescent beads, include spectra for comparison
    • Create more samples for MB/DNA with 100pM intervals from 1nM to 100pM, and a 75pM sample
    • Create more samples for Oligo D at 50, 30, and 10pM concentrations
    • For pM concentrations increase time to 700s to eliminate noise from spectra
    • Attach AuNPs in proper ratio at successfully run concentrations of MB/DNA



Personal tools