June 28, 2013
Notes from Meeting
- Find averages and standard deviation of inconsistent DNA/ThT spectra for calibration curve
- Fit Gaussian for inconsistent spectra (use Igor Pro program)
- Check literature for AuNP@SiO2 shift and silica-shell radius calculations
- Label axes for FCS data
- FCS spectra for fluorescent beads in prezi
- FCS: increase time as concentration decreases
- Repeat old AuNP procedure with solutions from new procedure
FCS Measurements
- Prepare Samples
- 10000x diluted green fluorescent beads
- 100, 80, 60, 20pM Oligo D
- 10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples
Results and Notes
Calibration graphs:<br.>
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- Oligo D samples were run for 300s, the 60pM and 80pM samples are still inconsistent with the trend of the other concentrations
- The first round of MB/DNA samples were run for 300s, results had lots of noise and inconsistencies
- The second round of MB/DNA samples were run for 700s to eliminate noise, only the 25pM sample lied outside the trend of the other concentrations
- Future work:
- Always align laser with 10000x diluted green fluorescent beads, include spectra for comparison
- Create more samples for MB/DNA with 100pM intervals from 1nM to 100pM, and a 75pM sample
- Create more samples for Oligo D at 50, 30, and 10pM concentrations
- For pM concentrations increase time to 700s to eliminate noise from spectra
- Attach AuNPs in proper ratio at successfully run concentrations of MB/DNA
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Meeting,FCS
