User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/01

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May 26, 2013

Coating AuNPs with Silica

from A General Method To Coat Colloidal Particles with Silica

Chemicals Needed

  • TES (tetraethyl orthosilicate)
  • PVP (Poly(vinylpyrrolidone))
  • Ethanol
  • Acetone
  • Ammonia (29.3 wt % NH3 in water)
  • Hydrofluoric acid

Procedure

Part One: Adsorption of PVP

  • Calculate the amount of PVP to provide the colloids with 60 PVP molecules per nm2
  • Dissolve the PVP in water by ultrasonication for 15 minutes
  • Combine the calculated amount of PVP with AuNPs and stir at 600 rpm for 24 hours at room temperature
  • Centrifuge solution and remove supernatant
  • Re-disperse the sediment in a solution of ammonia in ethanol

Part Two: Growth of the Silica Shell

  • Add an amount TES solution (10 vol % in ethanol) depending on the desired thickness of the silica shell, while stirring at 600 rpm
  • Stir reaction mixture for 12 hours

Calculations


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