User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==July 5, 2013== | ==July 5, 2013== | ||
===FCS Sample Prep=== | ===FCS Sample Prep=== | ||
*Green fluorescent beads | |||
**10000x diluted, run 3x for 90s | |||
*PBS | |||
**To check for background, run 3x for 200s | |||
*DNA/MB | |||
**125/100pM, run 3x for 500s | |||
**100/80pM, run 3x for 500s | |||
**75/60pM, run 3x for 500s | |||
**50/40pM, run 3x for 500s | |||
**25/20pM, run 3x for 500s | |||
===FCS Data=== | ===FCS Data=== | ||
Graphs generated using Igor Pro.<br.> | |||
<br.> | |||
[[Image:Avg_Beads.png]]<br.> | |||
10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> | |||
<br.> | |||
[[Image:Avg_PBS.png]]<br.> | |||
PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> | |||
[[Image:Avg_125pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_100pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_75pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_50pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_25pM.png]]<br.> | |||
All DNA/MB samples run three times, for 500s.<br.> | |||
<br.> | |||
Calibration Graph:<br.> | |||
[[Image:2013_0705_calibration.PNG]] | |||
Revision as of 13:49, 5 July 2013
FCS Measurements | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 5, 2013FCS Sample Prep
FCS DataGraphs generated using Igor Pro.<br.> <br.> <br.> 10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> <br.> <br.> PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> All DNA/MB samples run three times, for 500s.<br.> <br.> Calibration Graph:<br.>
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