User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Meeting, FCS Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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**Different diffusion times indicate background is something other than unbound DNA | **Different diffusion times indicate background is something other than unbound DNA | ||
*Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples | *Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples | ||
*Look in literature for ways to calculate silica shell thickness without TEM results | |||
===FCS Sample Prep=== | |||
*Green fluorescent beads | |||
**10000x diluted | |||
**Run 3x, 90s | |||
*Oligo D | |||
**150pM | |||
**125pM | |||
**100pM | |||
**Run 3x, 500s | |||
*DNA/MB: DNA held at a constant concentration to isolate background from DNA | |||
**600/500 | |||
**600/400 | |||
**600/300 | |||
**600/200 | |||
**600/100 | |||
**Run 3x, 500s | |||
Latest revision as of 22:58, 26 September 2017
Meeting, FCS Measurements | Main project page Previous entry Next entry |
July 8, 2013Notes from Meeting
FCS Sample Prep
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