User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/08: Difference between revisions
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==July 8, 2013== | ==July 8, 2013== | ||
===Notes from Meeting=== | ===Notes from Meeting=== | ||
* | *Since we're seeing some noise from just PBS on the FCS: make new PBS and run, parse out 10mL samples to avoid contaimination | ||
*Sonicate or centrifuge green fluorescent beads before running to prevent aggregation while measurements are being taken | |||
*Go up to 500pM for Oligo D samples (and lower concentrations) | |||
*Use a high, constant concentration of DNA with lower, decreasing concentration of MB | |||
*Check for consistent diffusion times between samples run on different days | |||
**Different diffusion times indicate background is something other than unbound DNA | |||
*Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples | |||
Revision as of 06:58, 8 July 2013
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July 8, 2013Notes from Meeting
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