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July 8, 2013
Notes from Meeting
- Since we're seeing some noise from just PBS on the FCS: make new PBS and run, parse out 10mL samples to avoid contaimination
- Sonicate or centrifuge green fluorescent beads before running to prevent aggregation while measurements are being taken
- Go up to 500pM for Oligo D samples (and lower concentrations)
- Use a high, constant concentration of DNA with lower, decreasing concentration of MB
- Check for consistent diffusion times between samples run on different days
- Different diffusion times indicate background is something other than unbound DNA
- Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples
- Look in literature for ways to calculate silica shell thickness without TEM results
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