User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/10

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July 10, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s per sample
  • Oligo D
    • 200pM
    • 175pM
    • 150pM
    • 125pM
    • 100pM
    • 75pM
    • 50pM
    • Run three times, 500s per sample

FCS Measurements