User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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*Take absorbance of final solution and three supernatants | *Take absorbance of final solution and three supernatants | ||
====Absorbance Data==== | ====Absorbance Data==== | ||
===Procedure Update: Silica-coating AuNPs by MHA=== | |||
We decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far. We also think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.> | |||
*Originally detailed on [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] | |||
*Day One: | |||
**Attach MHA to AuNPs: add 5mL of 10mM MHA to 5mL of AuNPs, stir overnight | |||
*Day Two: | |||
**Prepare the following solutions: | |||
#1mL 9.4nM, 10nm AuNP&MHA/5μL TES/0.125mL NH4OH/5mL propanol | |||
#1mL 9.4nM, 10nm AuNP&MHA/4μL TES/0.125mL NH4OH/5mL propanol | |||
#1mL 9.4nM, 10nm AuNP&MHA/3μL TES/0.125mL NH4OH/5mL propanol | |||
#1mL 9.4nM, 10nm AuNP&MHA/2μL TES/0.125mL NH4OH/5mL propanol | |||
#1mL 9.4nM, 10nm AuNP&MHA/1μL TES/0.125mL NH4OH/5mL propanol | |||
**Stir solutions for one hour to allow reaction to proceed | |||
**Centrifuge solutions at 4000 rpm for one hour | |||
**Re-suspend particles in water to a final concentration of 5nM? | |||
Revision as of 09:09, 12 July 2013
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July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
Absorbance DataProcedure Update: Silica-coating AuNPs by MHAWe decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far. We also think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.>
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