User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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*Day One: | *Day One: | ||
**Attach MHA to AuNPs: add 5mL of 10mM MHA to 5mL of AuNPs, stir overnight | **Attach MHA to AuNPs: add 5mL of 10mM MHA to 5mL of AuNPs, stir overnight | ||
***To prepare 10mM MHA solution: | |||
***(5 mL)(L/1000mL)(10mmoles/L)(1mol/1000mmoles)(288.49g/mol)=0.0144g MHA in 5mL | |||
*Day Two: | *Day Two: | ||
**Prepare the following solutions: | **Prepare the following solutions: |
Revision as of 06:48, 15 July 2013
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July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
NotesProcedure stopped because the nanoparticles could not be extracted by centrifugation. Absorbance data to be taken tomorrow. Procedure Update: Silica-coating AuNPs by MHAWe decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far, and we think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.>
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