User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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==July 12, 2013== | ==July 12, 2013== | ||
===Notes from Meeting=== | ===Notes from Meeting=== | ||
*Adjust range and use SymphoTime to better fit FCS graphs in Igor | |||
*Use error bars on calibration graphs (for Igor <N> values) | |||
*Focus on attaching AuNPs to DNA & hybridizing MB to run on FCS | |||
*Use a range of volumes of TES in MHA silica-coating procedure to change silica-coating thickness | |||
**Take absorbance data to see differences between solution, then use TEM imaging to verify presence of silica-coating | |||
===DNA and AuNP Attachment Procedure=== | ===DNA and AuNP Attachment Procedure=== | ||
====Calculations==== | ====Calculations==== | ||
Done by [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/12 Allison Alix]. | Done by [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/12 Allison Alix].<br.> | ||
Combine 231.3uL 1.03uM DNA, 231.3uL 29.67nM 14.7nm AuNPs, and 37.3uL sodium citrate buffer prepared 07/11/2013 <br.> | |||
Revision as of 08:39, 12 July 2013
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July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculationsDone by Allison Alix.<br.> Combine 231.3uL 1.03uM DNA, 231.3uL 29.67nM 14.7nm AuNPs, and 37.3uL sodium citrate buffer prepared 07/11/2013 <br.>
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