User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/16

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MHA Day Two</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MHA Day Two, Sample Preparation</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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===MHA Day Two Procedure===
===MHA Day Two Procedure===
Originally outlined on 05/24/2013, updated [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] <br.>
Originally outlined on 05/24/2013, updated [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] <br.>
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*Four samples of silica-coated nanoparticles made
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*Following samples of silica-coated nanoparticles made:
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**5uL TES, MHA
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**10uL TES, MHA
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**15uL TES, MHA
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**20uL TES, MHA
*TEM measurements to be taken at NIST on Thursday 07/18/2013
*TEM measurements to be taken at NIST on Thursday 07/18/2013
===Sample Prep: AuNP@SiO<sub>2</sub> Dilutions===
===Sample Prep: AuNP@SiO<sub>2</sub> Dilutions===
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All dilutions made in PBS.<br.>
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*Silica-coated AuNPs from 06/20/2013
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**5uL TES, MHA
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***Original
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***10x diluted
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***100x diluted
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***1000x diluted
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*Silica-coated AuNPs from today
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**5uL TES, MHA; 10uL TES, MHA; 15uL TES, MHA; 20uL TES, MHA
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***Original
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***10x diluted
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***100x diluted
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***1000x diluted
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*10nm AuNPs
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**Concentrations determined by [theoretical] resulting AuNP concentration in solution of silica-coating procedure
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**1nM
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**0.5nM
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Absorbance measurements of all samples to be taken tomorrow.<br.>

Current revision

MHA Day Two, Sample Preparation Main project page
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July 16, 2013

MHA Day Two Procedure

Originally outlined on 05/24/2013, updated 07/12/2013

  • Following samples of silica-coated nanoparticles made:
    • 5uL TES, MHA
    • 10uL TES, MHA
    • 15uL TES, MHA
    • 20uL TES, MHA
  • TEM measurements to be taken at NIST on Thursday 07/18/2013

Sample Prep: AuNP@SiO2 Dilutions

All dilutions made in PBS.

  • Silica-coated AuNPs from 06/20/2013
    • 5uL TES, MHA
      • Original
      • 10x diluted
      • 100x diluted
      • 1000x diluted
  • Silica-coated AuNPs from today
    • 5uL TES, MHA; 10uL TES, MHA; 15uL TES, MHA; 20uL TES, MHA
      • Original
      • 10x diluted
      • 100x diluted
      • 1000x diluted
  • 10nm AuNPs
    • Concentrations determined by [theoretical] resulting AuNP concentration in solution of silica-coating procedure
    • 1nM
    • 0.5nM

Absorbance measurements of all samples to be taken tomorrow.



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