User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/16

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(July 16, 2013)
 
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===MHA Day Two Procedure===
===MHA Day Two Procedure===
Originally outlined on 05/24/2013, updated [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] <br.>
Originally outlined on 05/24/2013, updated [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] <br.>
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*Four samples of silica-coated nanoparticles made
+
*Following samples of silica-coated nanoparticles made:
 +
**5uL TES, MHA
 +
**10uL TES, MHA
 +
**15uL TES, MHA
 +
**20uL TES, MHA
*TEM measurements to be taken at NIST on Thursday 07/18/2013
*TEM measurements to be taken at NIST on Thursday 07/18/2013
===Sample Prep: AuNP@SiO<sub>2</sub> Dilutions===
===Sample Prep: AuNP@SiO<sub>2</sub> Dilutions===
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+
All dilutions made in PBS.<br.>
 +
*Silica-coated AuNPs from 06/20/2013
 +
**5uL TES, MHA
 +
***Original
 +
***10x diluted
 +
***100x diluted
 +
***1000x diluted
 +
*Silica-coated AuNPs from today
 +
**5uL TES, MHA; 10uL TES, MHA; 15uL TES, MHA; 20uL TES, MHA
 +
***Original
 +
***10x diluted
 +
***100x diluted
 +
***1000x diluted
 +
*10nm AuNPs
 +
**Concentrations determined by [theoretical] resulting AuNP concentration in solution of silica-coating procedure
 +
**1nM
 +
**0.5nM
 +
Absorbance measurements of all samples to be taken tomorrow.<br.>

Current revision

MHA Day Two, Sample Preparation Main project page
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July 16, 2013

MHA Day Two Procedure

Originally outlined on 05/24/2013, updated 07/12/2013

  • Following samples of silica-coated nanoparticles made:
    • 5uL TES, MHA
    • 10uL TES, MHA
    • 15uL TES, MHA
    • 20uL TES, MHA
  • TEM measurements to be taken at NIST on Thursday 07/18/2013

Sample Prep: AuNP@SiO2 Dilutions

All dilutions made in PBS.

  • Silica-coated AuNPs from 06/20/2013
    • 5uL TES, MHA
      • Original
      • 10x diluted
      • 100x diluted
      • 1000x diluted
  • Silica-coated AuNPs from today
    • 5uL TES, MHA; 10uL TES, MHA; 15uL TES, MHA; 20uL TES, MHA
      • Original
      • 10x diluted
      • 100x diluted
      • 1000x diluted
  • 10nm AuNPs
    • Concentrations determined by [theoretical] resulting AuNP concentration in solution of silica-coating procedure
    • 1nM
    • 0.5nM

Absorbance measurements of all samples to be taken tomorrow.



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