User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/16: Difference between revisions

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===MHA Day Two Procedure===
===MHA Day Two Procedure===
Originally outlined on 05/24/2013, updated [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] <br.>
Originally outlined on 05/24/2013, updated [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] <br.>
*Four samples of silica-coated nanoparticles made
*Following samples of silica-coated nanoparticles made:
**5uL TES, MHA
**10uL TES, MHA
**15uL TES, MHA
**20uL TES, MHA
*TEM measurements to be taken at NIST on Thursday 07/18/2013
*TEM measurements to be taken at NIST on Thursday 07/18/2013
===Sample Prep: AuNP@SiO<sub>2</sub> Dilutions===
===Sample Prep: AuNP@SiO<sub>2</sub> Dilutions===
 
All dilutions made in PBS.<br.>
*Silica-coated AuNPs from 06/20/2013
**5uL TES, MHA
***Original
***10x diluted
***100x diluted
***1000x diluted
*Silica-coated AuNPs from today
**5uL TES, MHA; 10uL TES, MHA; 15uL TES, MHA; 20uL TES, MHA
***Original
***10x diluted
***100x diluted
***1000x diluted
*10nm AuNPs
**Concentrations determined by [theoretical] resulting AuNP concentration in solution of silica-coating procedure
**1nM
**0.5nM
Absorbance measurements of all samples to be taken tomorrow.<br.>




Revision as of 12:46, 16 July 2013

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July 16, 2013

MHA Day Two Procedure

Originally outlined on 05/24/2013, updated 07/12/2013 <br.>

  • Following samples of silica-coated nanoparticles made:
    • 5uL TES, MHA
    • 10uL TES, MHA
    • 15uL TES, MHA
    • 20uL TES, MHA
  • TEM measurements to be taken at NIST on Thursday 07/18/2013

Sample Prep: AuNP@SiO2 Dilutions

All dilutions made in PBS.<br.>

  • Silica-coated AuNPs from 06/20/2013
    • 5uL TES, MHA
      • Original
      • 10x diluted
      • 100x diluted
      • 1000x diluted
  • Silica-coated AuNPs from today
    • 5uL TES, MHA; 10uL TES, MHA; 15uL TES, MHA; 20uL TES, MHA
      • Original
      • 10x diluted
      • 100x diluted
      • 1000x diluted
  • 10nm AuNPs
    • Concentrations determined by [theoretical] resulting AuNP concentration in solution of silica-coating procedure
    • 1nM
    • 0.5nM

Absorbance measurements of all samples to be taken tomorrow.<br.>



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