User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/17

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Data, AuNP/DNA Attachment</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==July 17, 2013==
==July 17, 2013==
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===AuNP@SiO<sub>2</sub> Dilutions Absorbance Data===
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===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
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All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.<br.>
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[[Image:2013_0717_5uL_TES-MHA_1.PNG]]  [[Image:2013_0717_5uL_TES-MHA_2.PNG]]<br.>
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<br.>
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[[Image:2013_0717_10uL_TES-MHA.PNG]][[Image:2013_0717_15uL_TES-MHA.PNG]]<br.>
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<br.>
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[[Image:2013_0717_20uL_TES-MHA.PNG]]  [[Image:2013_0717_dilution_comparison.PNG]]<br.>
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<br.>
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===DNA and AuNP Attachment Procedure===
===DNA and AuNP Attachment Procedure===
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Replicate procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/15 07/15/2013] with 268mM sodium citrate buffer.
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====Absorbance Data====
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[[Image:2013_0717_AuNP-DNA_&_sups.PNG]]<br.>
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Corrected, with HEPES buffer used as reference solution.<br.>
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Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.<br.>
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===For NIST Tomorrow===
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*AuNP@SiO<sub>2</sub> samples in PBS and in water (26)
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*AuNP samples (3)
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*Notebook
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*Read over [http://www.nist.gov/customcf/get_pdf.cfm?pub_id=854083 Measuring the Size of Nanoparticles Using Transmission Electron Microscopy (TEM)]

Current revision

Absorbance Data, AuNP/DNA Attachment Main project page
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July 17, 2013

Absorbance Data: AuNP@SiO2 Dilutions

All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.
Image:2013_0717_5uL_TES-MHA_1.PNG Image:2013_0717_5uL_TES-MHA_2.PNG

Image:2013_0717_10uL_TES-MHA.PNGImage:2013_0717_15uL_TES-MHA.PNG

Image:2013_0717_20uL_TES-MHA.PNG Image:2013_0717_dilution_comparison.PNG

DNA and AuNP Attachment Procedure

Replicate procedure from 07/15/2013 with 268mM sodium citrate buffer.

Absorbance Data

Image:2013_0717_AuNP-DNA_&_sups.PNG
Corrected, with HEPES buffer used as reference solution.
Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.

For NIST Tomorrow



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