User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/17

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(Bring to NIST Tomorrow)
Current revision (14:04, 17 July 2013) (view source)
(Absorbance Data)
 
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==July 17, 2013==
==July 17, 2013==
===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
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All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.<br.>
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[[Image:2013_0717_5uL_TES-MHA_1.PNG]]  [[Image:2013_0717_5uL_TES-MHA_2.PNG]]<br.>
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<br.>
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[[Image:2013_0717_10uL_TES-MHA.PNG]][[Image:2013_0717_15uL_TES-MHA.PNG]]<br.>
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<br.>
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[[Image:2013_0717_20uL_TES-MHA.PNG]]  [[Image:2013_0717_dilution_comparison.PNG]]<br.>
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<br.>
===DNA and AuNP Attachment Procedure===
===DNA and AuNP Attachment Procedure===
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Replicate procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/15 07/15/2013] with 268mM sodium citrate buffer.
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====Absorbance Data====
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[[Image:2013_0717_AuNP-DNA_&_sups.PNG]]<br.>
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Corrected, with HEPES buffer used as reference solution.<br.>
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Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.<br.>
===For NIST Tomorrow===
===For NIST Tomorrow===
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*AuNP@SiO<sub>2</sub> samples (16)
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*AuNP@SiO<sub>2</sub> samples in PBS and in water (26)
*AuNP samples (3)
*AuNP samples (3)
*Notebook
*Notebook

Current revision

Absorbance Data, AuNP/DNA Attachment Main project page
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July 17, 2013

Absorbance Data: AuNP@SiO2 Dilutions

All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.
Image:2013_0717_5uL_TES-MHA_1.PNG Image:2013_0717_5uL_TES-MHA_2.PNG

Image:2013_0717_10uL_TES-MHA.PNGImage:2013_0717_15uL_TES-MHA.PNG

Image:2013_0717_20uL_TES-MHA.PNG Image:2013_0717_dilution_comparison.PNG

DNA and AuNP Attachment Procedure

Replicate procedure from 07/15/2013 with 268mM sodium citrate buffer.

Absorbance Data

Image:2013_0717_AuNP-DNA_&_sups.PNG
Corrected, with HEPES buffer used as reference solution.
Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.

For NIST Tomorrow



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