User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Data, AuNP/DNA Attachment</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Data, AuNP/DNA Attachment</span>
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==July 17, 2013==
==July 17, 2013==
===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.<br.>
[[Image:2013_0717_5uL_TES-MHA_1.PNG]]  [[Image:2013_0717_5uL_TES-MHA_2.PNG]]<br.>
<br.>
[[Image:2013_0717_10uL_TES-MHA.PNG]][[Image:2013_0717_15uL_TES-MHA.PNG]]<br.>
<br.>
[[Image:2013_0717_20uL_TES-MHA.PNG]]  [[Image:2013_0717_dilution_comparison.PNG]]<br.>
<br.>


===DNA and AuNP Attachment Procedure===
===DNA and AuNP Attachment Procedure===
Replicate procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/15 07/15/2013] with 268mM sodium citrate buffer.
====Absorbance Data====
[[Image:2013_0717_AuNP-DNA_&_sups.PNG]]<br.>
Corrected, with HEPES buffer used as reference solution.<br.>
Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.<br.>
===For NIST Tomorrow===
*AuNP@SiO<sub>2</sub> samples in PBS and in water (26)
*AuNP samples (3)
*Notebook
*Read over [http://www.nist.gov/customcf/get_pdf.cfm?pub_id=854083 Measuring the Size of Nanoparticles Using Transmission Electron Microscopy (TEM)]




Latest revision as of 23:04, 26 September 2017

Absorbance Data, AuNP/DNA Attachment Main project page
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July 17, 2013

Absorbance Data: AuNP@SiO2 Dilutions

All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.<br.> <br.> <br.> <br.> <br.> <br.> <br.>

DNA and AuNP Attachment Procedure

Replicate procedure from 07/15/2013 with 268mM sodium citrate buffer.

Absorbance Data

<br.> Corrected, with HEPES buffer used as reference solution.<br.> Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.<br.>

For NIST Tomorrow



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